Figure 1.

Citrullinated protein expression in the immunized skin of pGIA mice. (A) The clinical score of pGIA (means ± SEM), n = 5. (B) Skin sections were immunohistochemically stained with anti-modified citrulline (AMC) antibodies in pGIA or control mice. Hematoxylin was used as the counterstain. (C) Skin lysates from the pGIA mice and the control mice at days 0, 7, and 14 were subjected to Western blot analysis using anti-citrullinated histone 3 (CitH3) antibodies. Three mice were examined, and p values were calculated using ordinary one-way ANOVA with the post hoc Tukey test for multiple comparisons; (D) Skins were immunohistochemically stained with anti-CitH3 antibodies in the pGIA and control mice. Hematoxylin was used as the counterstain.