Figure 5.

JQ1 + gemcitabine inhibits the expression of HMGCS2 and APOC1 in PA4 and PA16 tumors. (A,B) qRT-PCR analysis for HMGCS2 (A), and APOC1 (B) mRNA expression in PA4 and PA16 tumors harvested from mice treated with vehicle (VC), JQ1, gem, or JQ1+gem. Data are presented as the mean ± SEM. One-way ANOVA followed by a Tukey post- hoc test were performed (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001). FFPE tumor sections of PA4 (C) and PA16 (D) were immunostained to detect HMGCS2 and APOC1 protein expression. Expression indices are shown in the lower left-hand corner of each photomicrograph and were calculated as described in Materials and Methods. Scale bar = 100 µm.