Figure 2.

Levels of inflammatory markers in BALF of WT and VAChT-KD^HOM mice. (A) Total cells; (B) Neutrophil; (C) IL-1β; and (D) CXCL-1/KC were measured in BALF of WT and VAChT-KD^HOM collected 24 h following intratracheal LPS instillation with (LPS+PNU groups) or without (LPS groups) treatment with PNU 282987 and compared to LPS-untreated WT and VAChT-KD^HOM mice (control groups) as described in Section 4.5 and Section 4.6. Data are expressed as mean ± SD of 5–9 mice per group. Significant differences are based on Holm–Šidak post hoc test following one-way ANOVA. Statistically significant differences versus LPS-untreated control group with the same genetic background are indicated with asterisks (** p < 0.01 and * p < 0.05), and significant differences between WT and VAChT-KD^HOM groups receiving the same experimental treatment are indicated in hashtags (# p < 0.05). The value obtained from each animal is represented by a circle.