Table 2.
Percent identity match of pigmented isolates to closest relative in the GenBank, accession numbers of isolates and culture attributes.
| Strain | Closest Relative in GenBank | Identity Match (%) | Accession |
X (g/L) |
TL (%) |
TC (µg/g) |
EPA (%) |
DHA (%) |
|---|---|---|---|---|---|---|---|---|
| RT2316-37 | Thraustochytrium sp. | 99.48 | MT812689 | 7.1 ± 0.3 b | 21.6 ± 0.8 b,c | 39.9 ± 1.3 c,d | 1.5 ± 0.0 b | 3.3 ± 0.4 b,c |
| RT2316-38 | Thraustochytrium sp. | 99.25 | MT812701 | 1.9 ± 0.1 d | 23.2 ± 1.2 b | 27.4 ± 5.3 e | 6.7 ± 1.1 a | 50.2 ± 2.7 a |
| RT2316-16 | Thraustochytrium sp. | 100.00 | MT648462 | 7.2 ± 0.2 b | 26.3 ± 1.7 a | 63.9 ± 3.2 a | 6.0 ± 0.0 a | 4.2 ± 0.0 b |
| RT2316-45 | Thraustochytrium sp. | 99.45 | MT814239 | 7.3 ± 0.1 b | 18.6 ± 1.2 c | 35.2 ± 6.6 d | 2.6 ± 1.5 b | 5.2 ± 1.5 b |
| RT2316-44 | Thraustochytrium sp. | 99.26 | MT814238 | 8.3 ± 0.2 a | 20.1 ± 1.3 b,c | 30.3 ± 2.7 d | 1.9 ± 0.0 b | 4.3 ± 0.4 b |
| RT2316-17 | Thraustochytrium sp. | 95.33 | MT648465 | 5.8 ± 0.3 c | 18.0 ± 0.3 c | 46.1 ± 3.2 c | 0.9 ± 0.5 b | 2.4 ± 1.2 c |
| RT2316-42 | Thraustochytrium sp. | 99.37 | MT812971 | 1.1 ± 0.1 f | 14.3 ± 2.1 d | 54.6 ± 1.7 b | 1.5 ± 0.5 b | 4.1 ± 0.0 b |
| RT2316-40 | Thraustochytrium sp. | 99.34 | MT812947 | 1.5 ± 0.1 e | 18.1 ± 0.5 c | 43.7 ± 1.3 c | 7.1 ± 0.0 a | 47.5 ± 0.0 a |
| RT2316-49 | Thraustochytriidae sp. | 99.47 | MT814293 | 8.3 ± 0.1 a | 18.5 ± 0.7 c | 40.5 ± 2.0 c,d | 1.6 ± 0.2 b | 4.1 ± 0.4 b |
| RT2316-50 | Aurantiochytrium sp. | 99.27 | MT814296 | 5.8 ± 0.3 c | 19.0 ± 0.2 c,d,e | ND | 0.8 ± 0.0 b | 2.7 ± 0.4 c |
X, Biomass concentration; TL, total lipid content in the dry biomass; TC, total carotenoid content in the dry biomass; EPA, percentage of EPA in fatty acids of total lipids; DHA, percentage of DHA in the fatty acids of the total lipids. All cultures were grown for 5 day at 15 °C. ND, Not detected. Different superscript letters within a column denote significant differences (p < 0.05).