Figure 1.

Cterm domain interacts with mutated mt-tRNALeu^(UUR) in MELAS cybrids. Relative quantification of mt-RNA species by RT-qPCR was achieved on the Cterm complexes immunoprecipitated with anti-FLAG antibodies from MELAS cybrids stably expressing FLAG-tagged Cterm. Values are referred to immunoprecipitated RNAs obtained from MELAS cybrids stably transfected with empty vector (mock), fixed as 1-value (dashed line). The amount of analysed mt-RNA species in the input was used as endogenous control. Results are presented as the mean ± S.D. Statistical analysis was performed on three independent biological replicates using two-tailed Student’s t test; asterisks (**, p < 0.01; ***, p < 0.001) indicate the significance of the enrichment of each analysed mt-RNA in Cterm-overexpressing cells with respect to mock. Individual data points are depicted by white diamond-shaped dots. Normal distribution of the six examined mt-RNAs was confirmed by the Shapiro–Wilk test; means were simultaneously compared by one-way ANOVA (α: 0.05). Lower table reports the post-hoc Tukey’s test p-values relative to compared pairs of samples (red: statistically significant; green: not statistically significant).