Figure 2.

(A) Cell viability analysis. Cells were treated with LGI-147 at the indicated concentrations for 72 h, and their viability was calculated using the trypan blue exclusion assay. (B) Activity of kinesin motor proteins. Purified Eg5, CENP-E, MKLP-1, and BimC underwent a cell-free kinesin ATPase end-point assay either under 50 pM of LGI-147 or not. Values shown are percentages of inhibition corrected by the controls. (C,D) Accumulation of abnormal mitotic cells (indicated by the arrows) due to LGI-147 treatment. After 24 h of treatment with the indicated concentrations of LGI-147, the HCC cells were fixed and stained with an anti-α-tubulin antibody (green). (C) Images shown are experiments on PLC5 cells captured through a fluorescence microscope. (D) Quantified results of Figure 2C.