Figure 5.
(A) Apoptosis due to Eg5 inhibition. Sub-G1 DNA content in HCC cells treated with vehicle or LGI-147 at the indicated concentrations for 72 h. The percentage of cells with sub-G1 DNA content is shown. Columns denote means, and bars denote standard deviations (n = 3). *, p < 0.05. (B,C) Suppression of in vivo tumor growth by LGI-147. PLC5 cells were injected subcutaneously into 5-week-old BALC/c mice (n = 6 per group). When tumor volume reached approximately 200 mm3, the mice were treated with intravenous injections of LGI-147 twice a week at the indicated doses. (B) Tumor size was estimated twice a week, and (C) body weight was monitored daily.