Figure 4.

MPE treatment downregulates antiapoptotic Bcl-2 family members in colon cancer cells. After 12 h and 48 h of incubation in the absence or presence of 360 μg/ml MPE, colon cell lines (HT29, Caco-2, and HCT116) were lysed and proteins were analyzed via Western blot, using specific antibodies for the proteins of interest. The blot of the γ-tubulin is also reported as a control of a homogeneous loading. The densitometry data were acquired using Quantity One software. For each set of data, the mean value of results obtained in three independent experiments was calculated and normalized to the housekeeping γ-tubulin protein. (*) p < 0.05, (**) p < 0.01, and (***) p < 0.001 versus untreated control.