Table 1.
New (this work) experimentally confirmed functional CDSs of bacteriophage TP-84 genome—an update 1.
| CDS Name | CDS Length (bp) | Location in the Genome (bp) | CDS Arbitrary Orientation | Polypeptide Length (aa) | Predicted Polypeptide Molecular Weight (kDa) | Experimentally Determined Polypeptide Molecular Weight (kDa) | Predicted Isoelectric Point | Hypothetical Function (Analysis) | Confirmed by Proteomic Analysis |
|---|---|---|---|---|---|---|---|---|---|
| TP84_01 | 567 | 13–579 | + | 188 | 22.4 | 22.4 | 7.76 | terminase, small subunit |
terminase, small subunit |
| TP84_02 | 1299 | 576–1874 | + | 432 | 50.0 | 50.1 | 7.74 | terminase, large subunit |
terminase, large subunit |
| TP84_04 | 354 | 2586–2939 | + | 117 | 13.6 | ND | 4.36 | unknown | unknown |
| TP84_15 | 324 | 10358–10681 | + | 107 | 11.9 | ND | 6.26 | unknown | unknown |
| TP84_20 | 375 | 12687–13061 | + | 124 | 14.7 | 14.7 | 9.80 | unknown | unknown |
| TP84_26 | 2976 | 20179–23154 | + | 991 | 112.2 | 112.9 | 5.49 | glycosylase | glycosylase |
| TP84_27 | 432 | 23240–23671 | + | 143 | 15.5 | 15.5 | 6.20 | holin | holin |
| TP84_30 | 573 | 25329–25901 | + | 190 | 21.7 | 21.7 | 4.98 | unknown | unknown |
| TP84_33 | 291 | 26597–26887 | + | 96 | 11.0 | ND | 5.10 | unknown | unknown |
| TP84_41 | 273 | 28824–29096 | + | 90 | 10.6 | ND | 9.69 | unknown | unknown |
| TP84_43 | 309 | 29330–29639 | + | 102 | 11.0 | ND | 9.81 | unknown | unknown |
| TP84_48 | 2265 | 30527–32791 | + | 754 | 86 | ND | 7.09 | unknown | unknown |
| TP84_49 | 474 | 32871–33344 | + | 157 | 18.0 | ND | 5.85 | unknown | unknown |
| TP84_58 | 318 | 37942–38259 | + | 105 | 12.3 | ND | 4.92 | replicative helicase inhibitor | replicative helicase inhibitor |
| TP84_59 | 1305 | 38259–39563 | + | 434 | 49.1 | 49.2 | 5.53 | replicative DNA helicase | replicative DNA helicase |
| TP84_60 | 672 | 39632–40303 | + | 223 | 26.4 | 26.4 | 8.41 | unknown | unknown |
| TP84_61 | 435 | 40281–40715 | + | 144 | 16.7 | ND | 9.08 | HNH homing endonuclease | HNH homing endonuclease |
| TP84_70 | 330 | 43704–44033 | + | 109 | 12.7 | ND | 9.47 | unknown | unknown |
| TP84_73 | 264 | 44746–45009 | + | 87 | 10.4 | ND | 5.60 | unknown | unknown |