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. 2021 Jul 7;12(7):1046. doi: 10.3390/genes12071046

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Comparative activity of the pEF1α promoter with that of the CAG promoter in regulating an immunomodulatory gene in different types of porcine cells. (A) Schematic of the cassettes for HLA-Ecw0304 and B2M (HLA-E) co-expression. Comparative expression of mRNA levels (B) and protein levels (C) of HLA-E in PEFs, -15 cells, and PAECs. pEF1α, porcine elongation factor 1α; HLA-E, human leukocyte antigen E; F2A, 2A self-cleaving peptides; B2M, β-2 microglobulin; p(A), poly A; Neo^®®, neomycin resistance. PEFs, porcine ear skin fibroblasts; PAECs, porcine aortic endothelial cells. ***, p < 0.001.