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. 2021 Jul 7;12(7):1046. doi: 10.3390/genes12071046

Figure 4.

Figure 4

The pEF1α promoter mediates significant increases in heterologous gene expression in response to primate serum in PAECs. (A) Luciferase activity, mRNA expression levels, and protein expression levels of HLA-E transgenes in PAECs transiently transfected with the expression cassettes presented in Figure 3A, followed by incubation in culture medium supplemented with pig serum. (B) Luciferase activity, mRNA expression levels, and protein expression levels of HLA-E transgenes in transfected PAECs incubated in culture medium supplemented with primate serum. (C–E) Expression pattern of endogenous pEF1α (C), TF (D), and ICAM2 (E) in PAECs cultured with porcine serum and primate serum for 1, 2, 3, 5, and 7 h. CAG, cytomegalovirus enhancer/chicken β-actin promoter; pEF1α, porcine elongation factor 1α; HLA, human leukocyte antigen E; ICAM2, intercellular adhesion molecule 2; TF, tissue factor. Values are means ± SEM calculated from three replicates. *, p < 0.05; **, p < 0.01; ***, p < 0.001.