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. 2021 Jul 7;12(7):1046. doi: 10.3390/genes12071046

Table 1.

Primers used in this study.

Experiment Gene Symbol Amplicon Size (bp) Primer (5′→3′)
Real-time PCR hHLA-E 501 GGGCTACCCGAGCCCGTCACCCTGAGATGG
TTCAATTCTCTCTCCATTCTTCAGTAAGTC
pGAPDH 219 TCGGAGTGAACGGATTTG
CCTGGAAGATGGTGATGG
pTF 181 AACTGAATGTGACCGTAGAAGCTG
CCTTTATCCACGTCAATCAGAAAC
pEF1α 136 TGGATTGCATTCTACCACCA
ACCATGCCAGGTTTGAGAAC
pICAM2 117 CGTGCTGCTCTTCTTGTTTG
CACCTCAGCCTCCTCTGAAC
Genotyping hHLA-E 1274 ATTTCCACACTTCCGTGTCC
TTCAATTCTCTCTCCATTCTTCAGTAAGTC

GAPDH, glyceraldehyde 3-phosphate dehydrogenase; HLA-E, human leukocyte antigen E; TF, tissue factor; EF1α, elongation factor 1α; ICAM2, intercellular adhesion molecule 2; h, human; p, pig.