Table 1.
Experiment | Gene Symbol | Amplicon Size (bp) | Primer (5′→3′) |
---|---|---|---|
Real-time PCR | hHLA-E | 501 | GGGCTACCCGAGCCCGTCACCCTGAGATGG |
TTCAATTCTCTCTCCATTCTTCAGTAAGTC | |||
pGAPDH | 219 | TCGGAGTGAACGGATTTG | |
CCTGGAAGATGGTGATGG | |||
pTF | 181 | AACTGAATGTGACCGTAGAAGCTG | |
CCTTTATCCACGTCAATCAGAAAC | |||
pEF1α | 136 | TGGATTGCATTCTACCACCA | |
ACCATGCCAGGTTTGAGAAC | |||
pICAM2 | 117 | CGTGCTGCTCTTCTTGTTTG | |
CACCTCAGCCTCCTCTGAAC | |||
Genotyping | hHLA-E | 1274 | ATTTCCACACTTCCGTGTCC |
TTCAATTCTCTCTCCATTCTTCAGTAAGTC |
GAPDH, glyceraldehyde 3-phosphate dehydrogenase; HLA-E, human leukocyte antigen E; TF, tissue factor; EF1α, elongation factor 1α; ICAM2, intercellular adhesion molecule 2; h, human; p, pig.