Figure 1.

Protective effects of Licochalcone D (Lico D) against hydrogen peroxide (H₂O₂). (A) Effect of Lico D on the cell viability was determined by MTT assay. (B) Protective effect of Lico D against H₂O₂ was confirmed by MTT assay. (C) ROS scavenging activity of Lico D was determined by DCFH-DA fluorescence assay and (D) fluorescence intensity was quantified using Image J software. (E) Representative images from immunoblot assay against apoptotic markers such as procaspase 3, Caspase 3 (cleaved form) and p53. GAPDH was used as an internal control. The scale bar represents 100 µm. All data are represented as the mean ± standard deviation (SD) (n = 3) * p < 0.05, ** p < 0.01, and *** p < 0.001.