Figure 8.
Expression of LGAL9 transcript variants in SKM-1, SKM-1/VCR, MOLM-13 and MOLM-13/VCR cells. (a) Representative gel (from 4 independent experiments) of RT-PCR detection of LGALS9 TVs in AML cell lines. All TVs (except X5) were amplified by the same pair of primers designed to produce PCR products with distinct molecular weights; (b–i)—densitometric quantification of respective PCR product bands obtained by RT-PCR of LGALS9 gene expression in respective cell types. Data represent the mean ± SEM of 4 independent experiments. Statistical significance is as follows: * p ≤ 0.05; ** p ≤ 0.02; *** p ≤ 0.001. MW—molecular weight marker; 1—SKM-1; 2—SKM-1/VCR; 3—MOLM-13; 4—MOLM-13/VCR.
