Figure 2.

Effect of 45 J/cm²–0.75 W laser treatment on pre-osteoblast proliferation. The frequency of irradiation was once per week (1× Week), three times (alternate day, 3× Week), and five times (every day, 5× Week). (A). The Hoechst stain assay revealed increased cell viability using both standard (ST) and flat-top (FT) hand-pieces. Notably, the FT device has managed to yet again increase the efficacy of the laser treatment at all time points applied (values represent means ± S.E., p < 0.05 compared to the control). (B–D). Single immunolabeling for c-myc (B) cyclin D3 (C) and CDK4 (D). The Fluorescence analysis was quantified by a Tecan Infinite fluorescence reader and values were analyzed by Magellan v4.0 software. Asterisks show the statistically significant differences of the samples analyzed compared with the control counterpart. Values represent means ± S.E.; * p < 0.05 (n = 4). Bar, 10 µm.