Figure 3.

Immunoblot analysis of extraocular muscle (EOM) versus diaphragm (DIA) muscle from wild type mice. Shown is an InstantBlue Coomassie stained protein gel using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) with molecular weight standards (lane 1; MWS), wild type EOM (lane 2) and wild type DIA (lane 3) samples, as well as identical nitrocellulose replicas used for immunoblotting (IB) and labelled with antibodies to myosin heavy chain MyHC14 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). In the adjacent panels are shown the statistical analysis of immunoblotting (Mann–Whitney U test; n = 8; ** p < 0.01). The value of molecular mass standards (×10⁻³ kDa) is marked on the left side of the gel.