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Comparison of alkaline phosphatase (ALP) activity at 7 d after calcification induction using MC3T3-E1 cells in normal (N), Collagen I-coated (Col I), and poly-L-lysine-coated (PLL) culture plates. ALP activity is expressed as Abs405/g protein (n = 3, * p < 0.05, ** p < 0.01, ## p < 0.01): * comparison among plates under the same media conditions; # comparison between two groups of the same type of nontreated (NT) and calcification (Cal) media in each plate.
