Behavioral analyses of miPiccolo mice. (A) The locomotor activity in a novel environment was measured every 5 min for 60 min (n = 12). * p < 0.05 vs. Mock-Saline, ##
p < 0.01 vs. miPiccolo-Saline (one-way ANOVA followed by Tukey’s post-hoc test). (B) Prepulse inhibition (PPI) was measured by presenting a semi-random series of prepulse of various intensities (74, 78, 82, 86 decibels) paired with the acoustic startle stimulus (120 db) to the mice (n = 12). * p < 0.05 vs. Mock-Saline, #
p < 0.05 vs. miPiccolo-Saline (one-way ANOVA followed by Tukey’s post-hoc test). (C) An indication of the time spent approaching the novel object in the trial phase of the novel object recognition test. Risperidone was administered 30 min before the acquisition and test phase (n = 12). *** p < 0.001 vs. Mock (one-way ANOVA followed by Tukey’s post-hoc test). (D) Spatial context learning was assessed based on the freezing time at 24 h after a conditioning session. Risperidone was administered 30 min before the training and test (n = 12). ** p < 0.01 vs. Mock (one-way ANOVA followed by Tukey’s post-hoc test). (E) Spatial working memory was assessed based on spontaneous alterations in the Y-maze test (n = 12). * p < 0.05 vs. Mock (one-way ANOVA followed by Tukey’s post-hoc test). Risperidone (0.01 mg/kg, i.p) was administered 30 min before the behavioral test.