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. 2021 Jul 5;10(7):1694. doi: 10.3390/cells10071694

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Senescence markers in N27 cells are regulated in nuclear (Nu) or cytosolic (Cy) fraction by α-syn PFF treatment. The α-syn PFF treatment reduces the levels of Lamin B1, HMGB1 and SATB1 in nuclear fractions, whereas it increases the levels of p16 in nuclear and p21 in cytosolic fractions after 48 h of exposure. The examples of cellular senescence markers are displayed in Western blots (A). The band intensities of Lamin B1 (B), HMGB1 (C), SATB1 (D), p16 (E) and p21 (F) were quantified using ImageJ. GAPDH and histone-3 were used as a cytosolic- and a nuclear-loading control, respectively. The relative levels (100% for vehicle treatment) are displayed in mean ± SEM and applied to unpaired Student’s t-test for statistical significance. **: p < 0.01, ***: p < 0.001 and ****: p < 0.0001.