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. 2021 Jul 5;10(7):1694. doi: 10.3390/cells10071694

Figure 5.

Figure 5

The levels of cellular senescence markers, such as LaminB1, HMGB1, SATB1 and p16, gradually decrease with α-syn PFF exposure, while the levels of p21 and GFAP increase in the isolated astrocyte culture over 3 days with α-syn PFF. In Western blots, cellular senescence markers were quantified in primary astrocytes culture at 12, 24, 48 and 72 h after α-syn PFF treatment to detect expression patterns over time (A). For assessing the expression patterns over 3 days of α-syn PFF exposure, we quantified the levels of Lamin B1 (B), HMGB1 (C), SATB1 (D), p16 (E), p21 (F) and GFAP (G). GAPDH was used as a loading control. The relative band intensities (100% for no PFF treatment) are displayed in mean ± SEM and applied to one-way ANOVA, Dunnett’s post-hoc test (#: compared with T = 0 point) for statistical significance. *: p <0.05, **: p < 0.01 and ***: p < 0.001.