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. 2021 Jul 5;10(7):1694. doi: 10.3390/cells10071694

Figure 11.

Figure 11

In cell-type-specific analyses in the CTX, the levels of Lamin B1 and HMGB1 are significantly lower in both astrocytes and microglia in α-syn PFF-injected brains than PBS-treated ones, whereas the levels of p21 in both cell types are increased with α-syn PFF. In the CTX, both GFAP (A–C) and Iba-1 (E–G) were increased in PFF-injected CTX (n = 6/group). The intensities of Lamin B1 and HMGB1 in both astrocytes (A,B,D) and microglia (E,F,H) were reduced; however, the levels of p21 were enhanced in both cell types with α-syn PFF (C,D,G,H). DAPI stain (blue) was also used to indicate the location of nucleus. The label intensities of Lamin B1, HMGB1 and p21 were quantified cell-type specifically, based on double-labels in a blinded manner. In quantification, PBS injected CTX region was used as a relative label intensity (100%, n = 5/group) in mean ± SEM and applied to unpaired Student’s t-test for statistical significance. ***: p < 0.001 and ****: p < 0.0001. Size bar: 100 µm.