The number of GFAP-positive astrocytes as well as the cell volume of astrocytes were significantly increased with α-syn PFF treatment in the STR, SNc and CTX. (A) The examples of enlarged astrocytes by α-syn PFF are compared with PBS treated controls and displayed in the STR, SNc and CTX. The astrocyte counting (B,D,F) and cell volume measurement (C,E,G) were analyzed in a blinded manner. In quantification, α-syn PFF-injected brains (n = 6/group) were compared with PBS-injected brains (n = 5/group) in the STR (B,C), SNc (D,E) and CTX (F,G), displayed as a relative number or volume (100% in PBS) in mean ± SEM and applied to unpaired Student’s t-test for statistical significance. ***: p < 0.001 and ****: p < 0.0001. Size bar: 100 µm.