| Let | = total transcript abundance (TA) of a gene in a WB sample | |
| = TA of a gene in B cells present inside the WB sample | ||
| = proportional cell count of B cells inside the WB sample (e.g., 5%) This value is assigned based on general knowledge or (in other settings) could be estimated from sample data by methods like deconvolution using TA data of other genes. |
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| = transcript abundance of all other cell-types based on their respective proportional counts. (This is a hypothetical parameter that is not actually determined or measured but is used to present a general model.) |
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| X | = fold change difference between and , = This is the observed difference in expression of a gene purified B cell samples and WB. |