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. 2021 Jun 25;12(7):971. doi: 10.3390/genes12070971
Let TAWB = total transcript abundance (TA) of a gene in a WB sample
TAB cells = TA of a gene in B cells present inside the WB sample
PB cells = proportional cell count of B cells inside the WB sample (e.g., 5%)
This value is assigned based on general knowledge or (in other settings) could be estimated from sample data by methods like deconvolution using TA data of other genes.
TAother cells = transcript abundance of all other cell-types based on their respective proportional counts.
(This is a hypothetical parameter that is not actually determined or measured but is used to present a general model.)
X = fold change difference between TAB cells and TAWB,
= TAB cellsTAWB
This is the observed difference in expression of a gene purified B cell samples and WB.