Table-1.

Primer sequences that are used in the real-time qPCR analysis

Gene	Sequence 5’-3’	Annealing temperature °C
β-actin	F: ATGTGGATCAGCAAGCAGGAGTA R: TTTATGCGCATTTATGGGTTTTGT	59
Claudin-1	F: CTGATTGCTTCCAACCAG R: CAGGTCAAACAGAGGTACAGG	56
Claudin-5	F: CATCACTTCTCCTTCGTCAGC R: GCACAAAGATCTCCCAGGTC	56
E-cadherin	F: GACAGGGACATGAGGCAGAA R: GCCGTGACAATGCCATTCTC	57
HSF1	F: CAGGGAAGCAGTTGGTTCACTACACG R: CCTTGGGTTTGGGTTGCTCAGTC	63
HSF3	F: TCCACCTCTCCTCTCGGAAG R: CAACAGGACTGAGGAGCAGG	57
HSP70	F: TCTCATCAAGCGTAACACCAC R: TCTCACCTTCATACACCTGGAC	55
HSP90	F: ATGCCGGAAGCTGTGCAAACACAGGACCAA R: GGAATCAGGTTAATTTTCAGGTCTTTTCCA	62
IL-6	F: GCTCGCCGGCTTCGA R: GGTAGGTCTGAAAGGCGAACAG	57
IL-8	F: CACGTTCAGCGATTGAACTC R: GACTTCCACATTCTTGCAGTG	59
Occludin	F: ACGGCAGCACCTACCTCAA R: GGGCGAAGAAGCAGATGAG	59
TLR-2	F: CCTGCAACGGTCACTTCAG R: GTCTCAGGGCTTGTTCTTCAG	58
TLR-4	F: CTGACCTACCCATCGGACAC R: GCCTGAGAGAGGTCAGGTTG	58