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Immunofluorescences of secondary antibody to anti-CD15s in control (A) and compound 1 treated cells (B). Notes: secondary antibody was conjugated with eFluor 660 fluorochrome. Fluorescences were measured in FL4 channel of flow cytometer for CD15s+ CSC, CD15s+CD44−, and CD15s+CD44+CD24+ cells. Abbreviations: CSC, cancer stem cells.
