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. 2021 Jul 9;22(14):7404. doi: 10.3390/ijms22147404

Figure 2.

Figure 2

The interactome of NCAM2; the mass spectrometry approach. (A) Schematic representation of the mass spectrometry approach. Brain lysates from P10–12 mice were enriched in membrane proteins and incubated with magnetic beads, which were previously conjugated with an antibody against NCAM2.1 or against both NCAM2 isoforms. The eluted proteins were processed for mass spectrometry analysis. A list of 103 proteins that interact with NCAM2 was obtained with a False Discovery Rate ≤ 0.01%, Table 1. (B) WB detection of NCAM2.1 and NCAM2 proteins in the cytosolic, membrane and insoluble fractions of the brain lysates used for the mass spectrometry assay. NCAM2.1 isoform is detected in both fractions, the membrane and the insoluble fraction while NCAM2.2 is specially enriched in the insoluble fraction. Tubulin was used as a loading control. (C) WB detection of NCAM2 from samples of magnetic beads elution.