Table 1.
The interactome of NCAM2.
| Protein (Gene) | Ptd | Function |
|---|---|---|
| Microtubule-associated protein 2 (Map2) | 24 | actin binding, calmodulin binding, cytoskeletal regulatory, microtubule binding and protein kinase binding |
| Neurofilament light polypeptide (Nefl) | 20 | structural constituent of cytoskeleton andstructural constituent of postsynaptic intermediate filament cytoskeleton |
| Actin, cytoplasmic 1 (Actb) | 19 | structural constituent of cytoskeleton and structural constituent of postsynaptic actin cytoskeleton |
| Neurofilament medium polypeptide (Nefm) | 19 | microtubule binding and structural constituent of cytoskeleton |
| Tubulin beta-4A (Tubb4a) | 18 | structural constituent of cytoskeleton and GTPase activity |
| Alpha-internexin (Ina) | 17 | structural constituent of cytoskeleton, structural constituent of postsynaptic actin and intermediate filament cytoskeleton |
| Tubulin alpha-1A (Tuba1a) | 13 | structural constituent of cytoskeleton |
| Tubulin alpha-1C (Tuba1c) | 12 | structural constituent of cytoskeleton |
| Actin, alpha cardiac muscle 1 (Actc1) | 11 | structural constituent of cytoskeleton and myosin binding |
| Calcium/calmodulin-dependent protein kinase type II beta (Camk2b) | 11 | calmodulin-dependent protein kinase activity, protein serine/threonine kinase activity and structural constituent of postsynaptic actin cytoskeleton |
| Calcium/calmodulin-dependent protein kinase type II alpha(Camk2a) | 10 | calmodulin-dependent protein kinase activity, glutamate receptor binding and protein serine/threonine kinase activity |
| Microtubule-associated protein 1B (Map1b) | 9 | actin binding, cytoskeletal regulatory protein and binding |
| Beta-actin-like protein 2 (Actbl2) | 8 | structural constituent of postsynaptic actin cytoskeleton |
| F-actin-capping protein beta (Capzb) | 8 | actin binding and beta-tubulin binding |
| 14-3-3 protein zeta/delta (Ywhaz) | 8 | recognition of a phosphoserine or phosphothreonine motif |
| Tubulin beta-6 chain (Tubb6) | 7 | structural constituent of cytoskeleton |
| Calcium/calmodulin-dependent protein kinase type II delta (Camk2d) | 6 | calmodulin-dependent protein kinase activity and protein serine/threonine kinase activity |
| Calcium/calmodulin-dependent protein kinase type II gamma (Camk2g) | 6 | calmodulin-dependent protein kinase activity and protein serine/threonine kinase activity |
| Enhancer of rudimentary homolog (Erh) | 5 | Cell cycle |
| Thioredoxin-dependent peroxide reductase (Prdx3) | 5 | cysteine-type endopeptidase inhibitor activity involved in apoptotic process |
| Heat shock cognate 71 kDa protein (Hspa8) | 5 | protein quality control system (folding proteins and degredation), co-chaperone, signaling receptor binding and ubiquitin protein ligase binding |
| F-actin-capping protein alpha-2 (Capza2) | 4 | actin filament binding |
| Calumenin (Calu) | 4 | calcium ion binding and enzyme inhibitor activity |
| Heat shock factor-binding protein 1 (Hsbp1) | 3 | transcription corepressor activity |
| Granulins (Grn) | 3 | chaperone binding, cytokine activity, growth factor activity and RNA binding |
| Reticulocalbin-2 (Rcn2) | 3 | calcium ion binding |
| Elongation factor 1-beta (Eef1b) | 3 | guanyl-nucleotide exchange factor activity and translation elongation factor activity |
| 14-3-3 protein epsilon (Ywhae) | 3 | recognition of a phosphoserine or phosphothreonine motif |
| Hemoglobin beta-1 (Hbb-b1) | 3 | oxygen carrier activity |
| Nuclease-sensitive element-binding protein 1 (Ybx1) | 3 | DNA-binding transcription activator, transcription factor binding |
| Elongation factor 1-delta (Eef1d) | 3 | activating transcription factor bindingand translation elongation factor activity |
| Dynein light chain 1, cytoplasmic (Dynll1) | 2 | motor activity and scaffold protein binding |
| Myosin light polypeptide 6 (Myl6) | 2 | motor activity and actin-dependent ATPase activity |
| Barrier-to-autointegration factor (Banf1) | 2 | DNA binding and enzyme binding |
| Dynein light chain 2, cytoplasmic (Dynll2) | 2 | motor activity and scaffold protein binding |
| 60S acidic ribosomal protein P2 (Rplp2) | 2 | structural constituent of ribosome |
| Complement component 1 Q (C1qbp) | 2 | complement component C1q binding, mRNA binding and translation activator |
| Inter-alpha-trypsin inhibitor heavy chain H3 (Itih3) | 2 | binding protein between hyaluronan and other matrix protein |
| Reticulon-4 (Rtn4) | 2 | cadherin binding and neurite growth regulatory factor |
| Ig kappa chain V-III region PC 2413 (Kv3a5) | 1 | adaptive immune response |
| Ataxin-10 (Atxn10) | 1 | neuritogenesis |
| Protein LSM12 homolog (Lsm12) | 1 | interactor/competitor of ATXN2 |
| Eukaryotic translation initiation factor 3 H (Eif3h) | 1 | translation initiation factor activity |
| Annexin A2 (Anxa2) | 1 | calcium-dependent phospholipid and cytoskeletal protein binding |
| Calmodulin (Calm1) | 1 | calcium-dependent protein and protein kinase binding |
| FAST kinase domain-containing protein 2 (Fastkd2) | 1 | protein kinase activity |
| Haptoglobin (Hp) | 1 | hemoglobin binding |
| Hippocalcin-like protein 1 (Hpcal1) | 1 | calcium ion binding |
| Ras-related protein Rab-33A (Rab33a) | 1 | GTPase activity |
| Ubiquitin-protein ligase E3B (Ube3b) | 1 | ubiquitin conjugating enzyme activity |
Proteins identified by LC-MS/MS in the NCAM2-immunoprecipitated cerebral cortex of postnatal mice samples obtained with a False Discovery Rate ≤ 0.01% and detected with two or more peptides. The table shows the proteins, the number of different peptides (Ptd) found from each protein and the function of the proteins.