Table 1.
Proposed functions of the products of genes encoded in the tobramycin biosynthetic gene cluster of Streptoalloteichus tenebrarius 2444 PS.
| Gene | Protein | BlastP Hits (Accession) |
Percent Identity (BlastP) | Putative Function | References for Functional Characterization |
|---|---|---|---|---|---|
| tobH1 (tacE) | TobH1 (tacE) | TobH1 (TacE) (CAH18547.1) |
100% | Unknown | - |
| YvrE (SHG81534.1) |
79.59% | Sugar lactone lactonase YvrE | - | ||
| tobX | TobX | TobX (CAH18549.1) |
100% | Unknown (dioxygenase) | - |
| tobE (tacD) | TobE (TacD) | TobE (Q2MF22.1) |
100% | 2-deoxy-scyllo-inosamine dehydrogenase (DOIA dehydrogenase) | - |
| tobT (tbmE) | TobT (TbmE) | TbmE (CAE22476.1) |
100% | Major facilitator superfamily protein | - |
| tobB (tacC) | TobB (TacC) | Hypothetical protein (CAE22475.1) |
100% | Unknown | - |
| Hypothetical protein (WP_073480773.1) |
86.84% | Aminotransferase class III-fold pyridoxal phosphate-dependent enzyme | - | ||
| tobQ (tacB) | TobQ (TacB) | Hypothetical protein (CAE22474.1) |
100% | 6′-dehydrogenase | [22] |
| Hypothetical protein (WP_073480774.1) |
89.37% | GMC family oxidoreductase | - | ||
| tobZ (tacA) | TobZ (TacA) | TacA (CAE22473.1) (Q70IY1.1) |
100% | O-carbamoyltransferase | [10,23] |
| tobS1 (tbmB) | TobS1 (TbmB) | TbmB (Q2MF17.1) |
100% | 2-deoxy-scyllo-inosose aminotransferase (l-glutamine:DOI aminotransferase) | [24,25] |
| tobC (tbmA) | TobC (TbmA) | TbmA (Q2MF16.1) |
100% | 2-deoxy-scyllo-inosose synthase (DOI synthase) | [14,25] |
| tobD2 (tbmC) | TobD2 (TbmC) | TbmC (CAE22470.1) |
100% | Dehydrogenase | - |
| tobM2 (tbmD) | TobM2 (TbmD) | TbmD (CAE22469.1) | 100% | 6-glycosyltransferase | [16] |
| tobN (tbmX) | TobN (TbmX) | TobN (CAH18559.1) | 100% | Amidase | - |
| tobS2 | TobS2 | TobS2 (Q2MF12.1) |
100% | l-glutamine:3-amino-2,3-dideoxy-scyllo-inosose aminotransferase (l-glutamine:amino-DOI aminotransferase) | - |
| tobH2 | TobH2 | Hypothetical protein (CAH18561.1) |
100% | Retrotransposon protein (possibly non-functional) | - |
| tobM1 | TobM1 | TobM1 (CAH18562.1) | 99.76% | Aminoglycoside 4-glucosaminyltransferase | [16] |
| tobH3 | TobH3 | No hits | - | Unknown | - |
| tobH4 | TobH4 | No hits | - | Unknown | - |
| tobL | TobL | TobL (CAH18563.1) | 100% | Carbamoyl-phosphate synthase | - |