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. 2021 Jul 13;22(14):7501. doi: 10.3390/ijms22147501

Figure 5.

Figure 5

NPD-0614-13 and NPD-0614-24 counteracted the TNF-α or LPS induced expression of pro-inflammatory cytokines in NHKs. (a) Quantitative real time PCR analysis of IL-6 and IL-8 in NHKs treated with TNF-α (20 ng/mL) or LPS (10 μg/mL) in the presence or absence of NPD-0614-13, NPD-0614-24 (25 μM) and FICZ (100 nM) for 6 h. All mRNA values were normalized against the expression of GAPDH and were expressed relative to untreated control cells. (b) IL-6 and IL-8 quantitation by ELISA in NHKs treated as above for 24 h. The data are expressed as mean ± SD of three independent experiments (significance vs. untreated control or vs. stimulated cells are marked with * and °, respectively; * p < 0.05, ** p < 0.01 vs. untreated control; ° p < 0.05 vs. TNF-α or LPS stimulated cells).