. 2021 Jul 2;10(7):1667. doi: 10.3390/cells10071667

Table 7.

Genes that have been engineered to improve the secretory machinery of the HEK293 cell line.

Gene	Function	Modification	Outcome	Recombinant Protein	Reference
SNARE receptor coding genes, *SNAP-23* and *VAMP8*	Mediate/catalyze the fusion of vesicles to target membranes during exocytosis	Overexpression	Improved recombinant protein expression	SEAP, SAMY	[110]
SM protein coding genes MUNC18 and sly1	Regulate SNARE complex assembly and then cooperate with SNAREs to stimulate membrane fusion.	Overexpression	Improved recombinant protein expression	SEAP, SAMY	[110]
Tetraspanin *CD9*	Play a role in many cellular processes including exosome biogenesis.	Overexpression	Up to 26% improvement in virus production	Exosome-Associated AAV	[109]