Figure 1.

Preparation of adult primary microglia cell cultures. (A) Co-immunostaining of PMCs for microglia (CD11b, green) and astrocytes (GFAP, red), oligodendrocytes (Olig2, red), or neurons (NeuN, red). Cell nuclei are stained with DAPI (blue). Images are representatives of three independent cultures, each from an individual control animal. Scale bars = 25 μm. (B) Representative Western blots (left) and densitometric analysis of Iba1 expression levels (right) in CT-PMCs and 22L-PMCs normalized per expression of β-actin. Data are expressed as mean ± SEM, n = 3 independent cultures isolated from individual animals, ** p < 0.01 (two-tailed, unpaired student t-test).