Figure 2.

Phosphorylation down-regulates Puf1 and Puf2 function. (A) Top, overnight cultures of WT (BY4741), and derived puf1∆ (yHG5), puf2∆ (yHG4), and puf1∆ puf2∆ (yHG2) cells, were serially diluted 10-fold and plated onto YPD containing solvent alone (-) or myriocin (Myr) at the indicated concentration. Pictures were taken after two days of growth. Middle, overnight cultures of WT, puf1∆ cells (yHG5), or cells expressing Puf1-6HA (yHG15), Puf1^AA-6HA (yHG16) or Puf1^EE-6HA (yHG22), as indicated, were serially diluted 10-fold and plated onto YPD containing solvent alone (-) or myriocin (Myr) at the indicated concentration. Pictures were taken after two days of growth. Bottom, overnight cultures of WT, puf2∆ cells (yHG4), or cells expressing Puf2^WT-HA (yHG17) or Puf2^AA-HA (yHG18), as indicated, were serially diluted 10-fold and plated onto YPD containing solvent alone (-) or myriocin (Myr) at the indicated concentration. (B) Same as in (A), except the cells were exposed to solvent alone (-) or aureobasidin A (AbA) at the indicated concentration.