Table 1.
S. cerevisiae strains used in this study.
| Scheme | Genotype | Source or Reference |
|---|---|---|
| BY4741 | MATahis3∆1 leu2∆0 ura3∆0 met15∆0 | Research Genetics, Inc. |
| BY4742 | MATα his3∆1 leu2∆0 ura3∆0 lys2∆0 | Research Genetics, Inc. |
| yAM135-A | BY4741 Ypk1(L424A)::ura3- ypk2∆::KanMX4 | [13] |
| yHG2 | BY4742 puf1∆::KanMX puf2∆::KanMX | Research Genetics, Inc. |
| yHG4 | BY4742 puf2∆::KanMX | Research Genetics, Inc. |
| yHG5 | BY4742 puf1∆::KanMX | This study |
| yHG8 | BY4742 Puf1-mKate2::SpHIS5 | This study |
| ySP1 | BY4742 Puf1(T174A S275A)-mKate2::SpHIS5 | This study |
| ySP8 | BY4742 Puf2-6HA-eGFP::SpHIS5-LEU2 | This study |
| ySP9 | BY4742 Puf2(S53A S54A S55A T56A T143A S246A S902A)-6HA-eGFP::SpHIS5-LEU2 | This study |
| yHG15 | BY4742 Puf1-6HA::LEU2 | This study |
| yHG16 | BY4742 Puf1(T174A S273A S275A)-6HA::LEU2 | This study |
| yHG22 | BY4742 Puf1(T174E S273E S275E)-6HA::LEU2 | This study |
| yHG17 | BY4742 Puf2-6HA::LEU2 | This study |
| yHG18 | BY4742 Puf2(S53A S54A S55A T56A T143A S246A S902A)-6HA::LEU2 | This study |
| YFR694 | BY4741 Puf1-6HA::LEU2 lys2∆0 MET15 | This study |
| YFR695 | BY4741 Puf1-6HA::LEU2 Ypk1(L424A)::URA3 ypk2∆::KanMX4 | This study |
| YFR699 | BY4741 Puf2-6HA::LEU2 | This study |
| YFR700 | BY4741 Puf2-6HA::LEU2 Ypk1(L424A)::URA3 ypk2∆::KanMX4 | This study |
| ySP18 | BY4742 Puf1::LEU2 Puf2-6HA::LEU2 Pmp3-3XFLAG met15∆0 | This study |
| ySP19 | BY4742 Puf1(T174A S273A S275A)::LEU2 Puf2(S53A S54A S55A T56A T143A S246A S902A)-6HA::LEU2 Pmp3-3XFLAG | This study |
| YFR205 | BY4741 fpk1∆::KanMX fpk2∆::KanMX lys2∆0 | [33] |
| YFR739-A | BY4742 Puf1::LEU2 Puf2-6HA::LEU2 met15∆0 | This study |
| YFR738 | BY4742 Puf1(T174A S273A S275A)::LEU2 Puf2(S53A S54A S55A T56A T143A S246A S902A)-6HA::LEU2 | This study |