pAX50 |
pBG1805 GAL1prom-Ypk1(L424A), URA3
|
[13] |
pGEX4T-1 |
GST tag, bacterial expression vector, AmpR
|
GE Healthcare, Inc. |
pHG10 |
pGEX4T-1 Puf1(133-220) |
This study |
pHG11 |
pGEX4T-1 Puf1(228-310) |
This study |
pHG12 |
pGEX4T-1 Puf2(6-90) |
This study |
pHG13 |
pGEX4T-1 Puf2(860-938) |
This study |
pHG18 |
pGEX4T-1 Puf1(133-220) (T174A) |
This study |
pHG19 |
pGEX4T-1 Puf1(228-310) (S273A S275A) |
This study |
pHG20 |
pGEX4T-1 Puf2(6-90) (S53A S54A S55A T56A) |
This study |
pHG21 |
pGEX4T-1 Puf2(860-938) (S902A) |
This study |
pKS137 |
CEN, PGK1prom-λN-1XFLAG-BFP::SpHIS5 |
[44] |
pHG32 |
pKS137-Puf1-λN-1XFLAG-BFP |
This study |
pHG33 |
pKS137-Puf1(T174A S275A)-λN-1XFLAG-BFP |
This study |
pHG35 |
pKS137-Puf1(1-340)-λN-1XFLAG-BFP |
This study |
pHG36 |
pKS137-Puf1(1-340) T174A S275A-λN-1XFLAG-BFP |
This study |
pNTI473 |
pPGK1::mCherry::(PP7)3
|
[44] |
pGF-V789 |
pRS316-GALprom-Cas9 |
[45] |
pSP9 |
pRS423-sgRNAPMP3
|
This study |