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. 2021 Jun 30;11(7):500. doi: 10.3390/membranes11070500

Table 2.

Plasmids used in this study.

Plasmid Description Source/Reference
pAX50 pBG1805 GAL1prom-Ypk1(L424A), URA3 [13]
pGEX4T-1 GST tag, bacterial expression vector, AmpR GE Healthcare, Inc.
pHG10 pGEX4T-1 Puf1(133-220) This study
pHG11 pGEX4T-1 Puf1(228-310) This study
pHG12 pGEX4T-1 Puf2(6-90) This study
pHG13 pGEX4T-1 Puf2(860-938) This study
pHG18 pGEX4T-1 Puf1(133-220) (T174A) This study
pHG19 pGEX4T-1 Puf1(228-310) (S273A S275A) This study
pHG20 pGEX4T-1 Puf2(6-90) (S53A S54A S55A T56A) This study
pHG21 pGEX4T-1 Puf2(860-938) (S902A) This study
pKS137 CEN, PGK1prom-λN-1XFLAG-BFP::SpHIS5 [44]
pHG32 pKS137-Puf1-λN-1XFLAG-BFP This study
pHG33 pKS137-Puf1(T174A S275A)-λN-1XFLAG-BFP This study
pHG35 pKS137-Puf1(1-340)-λN-1XFLAG-BFP This study
pHG36 pKS137-Puf1(1-340) T174A S275A-λN-1XFLAG-BFP This study
pNTI473 pPGK1::mCherry::(PP7)3 [44]
pGF-V789 pRS316-GALprom-Cas9 [45]
pSP9 pRS423-sgRNAPMP3 This study