Table 2.
Primers of reference genes in T. absoluta.
| Gene | Primer Sequence (5′–3′) | Product Size (bp) | E (%) | R2 |
|---|---|---|---|---|
| EF1α | F: CCTGGGCACAGAGATTTCAT R: GATCAGCTGCTTGACACCAA |
171 | 98.3 | 0.997 |
| AK | F: GCCCAGTACAAGGAGATGGA R: ACCACACGAGGAAGGTCTTG |
238 | 99.3 | 0.998 |
| SOD | F: GGGCCTCATTTCATTGCTTA R: CTTCGCCACTGCTTATAGCC |
190 | 109.1 | 0.996 |
| ACT | F: GCGACATCAAGGAGAAGCTC R: CAAGCTTCCATACCCAGGAA |
187 | 97.2 | 0.991 |
| GAPDH | F: GCGTCAACCTTGAAGCCTAC R: TTACCAGAGGGACCGTCAAC |
181 | 102 | 0.993 |
| RPL7A | F: TCAACCAGTTCACCCAGACA R: CACGAGCTGAGCCTTCTTCT |
227 | 100.8 | 0.995 |
| RPL10 | F: CTTCATCCCTTCCACGTCAT R: TGAAACCCCACTTCTTGGAC |
250 | 95.6 | 0.998 |
| RPL28 | F: TCAGACGTGCTGAACACACA R: GCCAGTCTTGGACAACCATT |
185 | 92.9 | 0.995 |
| RPS11 | F: AAGACCTGCCGATATGCAAC R: TAGCCGTAGTCTGAGCAGCA |
156 | 98.4 | 0.994 |
E, RT-qPCR efficiency; R2, regression coefficient; F, forward primer; R, reverse primer.