Table 3.

Ranking of the T. absoluta reference genes under four experimental conditions.

Biotic Conditions	Rank	geNorm		Normfinder		BestKeeper		ΔCt
		Gene	Stability	Gene	Stability	Gene	Stability	Gene	Stability
Developmental stage	1	GAPDH	1.654	RPS11	0.110	RPL28	0.785	RPL28	0.889
	2	EF1 α	1.669	GAPDH	0.189	RPS11	1.001	RPL10	0.916
	3	RPS11	1.776	RPL28	0.240	GAPDH	1.129	ACT	0.944
	4	RPL28	1.781	EF1 α	0.576	EF1α	1.422	EF1 α	0.976
	5	ACT	1.875	ACT	0.901	ACT	1.526	RPS11	0.979
	6	RPL10	2.078	RPL10	1.131	AK	1.643	AK	0.987
	7	RPL7A	2.354	RPL7A	1.424	RPL10	2.016	RPL7A	1.025
	8	AK	3.187	AK	1.845	RPL7A	2.386	GAPDH	1.031
	9	SOD	4.567	SOD	2.110	SOD	2.797	SOD	1.359
Tissues	1	RPL28	1.343	RPL28	0.235	RPL28	0.779	RPL28	0.825
	2	RPL10	1.348	RPL10	0.385	GAPDH	1.023	RPL10	0.912
	3	RPL7A	1.364	RPL7A	0.435	RPL7A	1.033	ACT	0.924
	4	GAPDH	1.668	GAPDH	0.772	RPL10	1.121	RPS11	0.955
	5	ACT	1.697	ACT	0.829	EF1α	1.206	EF1α	0.961
	6	EF1 α	1.823	EF1 α	1.013	SOD	1.250	AK	0.982
	7	AK	1.990	AK	1.106	Actin	1.503	RPL7A	1.013
	8	RPS11	2.184	RPS11	1.287	AK	1.656	GAPDH	1.032
	9	SOD	2.205	SOD	1.297	RPS11	1.909	SOD	1.264
20E	1	EF1 α	0.651	EF1 α	0.161	GAPDH	0.440	RPL28	0.946
	2	RPL28	0.710	RPL28	0.235	RPL28	0.496	RPL10	0.969
	3	RPL7A	0.741	AK	0.280	AK	0.510	ACT	0.973
	4	AK	0.753	RPL7A	0.342	EF1α	0.628	RPS11	0.991
	5	RPS11	0.852	SOD	0.473	RPS11	0.923	EF1α	0.995
	6	SOD	0.900	RPS11	0.493	SOD	1.234	AK	0.995
	7	RPL10	0.906	RPL10	0.502	RPL7A	1.316	RPL7A	1.003
	8	GAPDH	0.989	GAPDH	0.630	ACT	1.378	GAPDH	1.020
	9	ACT	0.989	ACT	0.630	RPL10	1.419	SOD	1.097
Insecticide	1	EF1 α	1.303	EF1 α	0.391	ACT	0.440	RPL28	0.819
	2	RPL7A	1.350	RPL7A	0.559	RPL7A	0.496	RPS11	0.959
	3	RPS11	1.474	AK	0.675	EF1α	0.510	RPL10	0.968
	4	AK	1.490	RPS11	0.734	RPL10	0.628	ACT	0.987
	5	GAPDH	1.545	SOD	0.795	SOD	0.923	EF1α	0.988
	6	ACT	1.558	ACT	0.844	AK	1.234	AK	0.990
	7	SOD	1.569	GAPDH	0.877	RPS11	1.316	RPL7A	1.005
	8	RPL28	1.728	RPL28	1.054	GAPDH	1.378	GAPDH	1.057
	9	RPL10	1.755	RPL10	1.057	RPL28	1.419	SOD	1.106