Figure 7.

Assessment of barrier damage from the exposure to a benchmark irritant (0.2% SDS). (a) TEER measurements performed with concentric electrodes integrated in the BoC device and an EVOM system. Measurements were performed over time before and after exposure to 0.2% SDS in PBS (at time = 0) for 3 batches of FTSm grown for 14 days on chip. (b) Assessment of barrier resistance in FTSm: representative fluorescence (top) and H&E images (bottom) cultured for 14 days at ALI and exposed to 0.2% SDS for 0 (control), 1 and 3 h, followed by the application of the green fluorescent dye lucifer yellow (LY, green) for 30 min to assess barrier integrity. Cell nuclei were stained by DAPI (blue). Scale bars: 100 μm.