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. 2021 Jul 2;10(7):1660. doi: 10.3390/cells10071660

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of NLRP3 triggers on cytosolic LCN2 levels. (A) BMDM were primed with LPS and then treated with NG or dsDNA. The cellular lysates were subjected to immunoblotting as indicated. (B) LPS-primed BMDM were treated with an inflammasome trigger as indicated, and then the cellular lysates were analyzed by IL-1β or LCN2 ELISA. (C) LPS-primed BMDM were treated with inflammasome triggers as indicated, and the levels of IL-1β, TNFα, and IL-6 in the lysate were measured. The bar graph shows the mean ± SD of at least two independent experiments.