Table 5.
Comparison of the sensitivity and specificity of RT-RPA in detecting different molecular targets of MERS-CoV and SARS-CoV-2.
| Virus type | Molecular targets | Sensitivity (SN) | Specificity (SP) | References |
|---|---|---|---|---|
| MERS-CoV | N | LOD: 10 RNA molecules (as sensitive as qRT-PCR) | 100% | [96] |
| N, UpE | UpE: LOD of 12 RNA copies/μL (86%) | UpE: 100% | [117] | |
| N: 100% | ||||
| N: LOD of 1.2 RNA copies/μL (100%) | ||||
| UpE, ORF 1a | LOD: 3.7-1 PFU of MERS-CoV | 100% | [118] | |
| UpE: 98.06% (compared with qRT-PCR) | ||||
| ORF 1a: 99.03% (compared with qRT-PCR) | ||||
| SARS-CoV-2 | N | LOD: 7.8 RNA molecules/reaction (100% concordance to qRT-PCR) | 100% | [120] |
| N | LOD: 5 RNA copy/μL sample (98%) | 100% | [119] | |
| N, S | LOD: 0.05 RNA copy/μL sample | Unclear | [97] | |
| ORF 1ab | 96.8% compared to qRT-PCR | Unclear | [121] | |
| N, RdRp | 2.5 RNA copies/μL input | 100% | [122] | |
| For qRT-PCR: LOD was 1 RNA copies/μL input | ||||
| For RT-LAMP: LOD was 10 RNA copies/μL input | ||||
| N, E, ORF 1ab | LOD: 1 RNA copy/μL sample (97%) | 100% | [323] | |
| N, E, RdRp | LOD: 2–15 RNA molecules/reaction (65–94%) | 77–100% | [124] |