Table 7.
Comparison of the sensitivity and specificity of CRISPR/Cas-based tests in detecting different molecular targets of MERS-CoV and SARS-CoV-2.
| Virus type | Molecular targets | Sensitivity (SN) | Specificity (SP) | References |
|---|---|---|---|---|
| MERS-CoV | N, E, S, M, ORF 1ab, RdRp | No data | 100% (no cross-reactivity with other pathogens like SARS-CoV-2) | [145], [146], [147], [148], [149] |
| SARS-CoV-2 | N | 95% concordance to qRT-PCR results | 100% | [150] |
| N | LOD: few RNA copies/sample | 100% | [146] | |
| S | LOD: 10 RNA copies/reaction(96.23% compared to RT-RPA) | 100% | [151] | |
| RdRp | LOD: 1 × 104 RNA copies/mL | 100% | [145] | |
| N, E | LOD: ~25 RNA copies/μL (93.1%) | 98.5% | [152] | |
| N, E | LOD: ~80 RNA copies/sample | <100% (minor cross-reactivity with other pathogens) | [155] | |
| N, ORF 1ab | LOD: 6.75 RNA copies/μL | 100% | [325] | |
| N, ORF 1ab | LOD: 1–10 RNA copies/reaction (100% consistent result with qRT-PCR) | 100% | [149] | |
| N, ORF 1ab | LOD: 7.5–25 RNA copies/μL | 100% | [326] | |
| E, ORF 1ab | LOD: 4 RNA copies/μL (100% -ve predictive agreement, 97.14% +ve predictive agreement) | 100% | [154] | |
| N, S, M | LOD: 0.1 RNA copies/μL | 100% | [153] | |
| Unclear | LOD: ~100 RNA copies/mL | 100% | [147] |