Table 8.
Comparison of the sensitivity and specificity of PCR-MS-based tests in detecting different molecular targets of MERS-CoV and SARS-CoV-2.
| Virus type | Molecular targets | Sensitivity (SN) | Specificity (SP) | References |
|---|---|---|---|---|
| MERS-CoV | N, UpE, RdRp, ORF 1b | LOD: 10–100 RNA copies/reaction | 100% | [156] |
| SARS-CoV-2 | N, ORF 1ab | Results 100% concordance to qRT-PCR | Specific and no false positive/negative results | [157] |
| N, ORF 1ab | LOD: 1562.5 RNA copies/mL (90–100%) | Unclear | [160] | |
| N, ORF 1ab | LOD: 400 RNA copies/mL | Unclear | [158] | |
| N, ORF 1ab | Detection rate: 75% | Unclear | [159] | |
| N, ORF 1ab | LOD: 0.34–110 RNA copies/μL | <100% (cross-reactive with other respiratory pathogens) | [161] |