Table 9.
Comparison of the sensitivity and specificity of different rapid, extraction-free molecular test kits/protocols in detecting SARS-CoV.
| Name of test kits (manufacturer/country) | Detection technology | Targets | Sensitivity | Specificity | References |
|---|---|---|---|---|---|
| Advanta Dx SARS-CoV-2 RT-PCR assay (Fluidigm) | RT-PCR | N | LOD: 6.25 RNA copies/μL | 100% | [171] |
| qScript ® XLT qRT-PCR (Quantabio) | RT-PCR | N | LOD: 6–12 RNA/μL(sensitivity: 85%) | Unclear | [173] |
| VereRT™ ZeroPrep™ COVID-19 PCR Kit (Veredus) | RT-PCR | N | LOD: 2 RNA copies/reaction | Unclear | [172] |
| XFree™ COVID-19 qRT-PCR test (BioGX) | RT-PCR | N | >90% | >90% | [170] |
| FastPlex™ Triplex SARS-CoV-2 Detection Kits | RT-PCR | N | 97.9% | 95.7% | [174] |
| SalivaDirect (Yale) | RT-PCR | N | LOD: 1.5 RNA copies/μL | 100% | [167] |
| Xpert ® Xpress (Cepheid) | RT-PCR | N, E | LOD: 0.005–0.02 PFU/mL | 100% | [166] |
| SARS-CoV-2 SANSURE®BIOTECH Novel Coronavirus (Sansure). | RT-PCR | N, ORF 1ab | 69.9–94.6% | 100% | [169] |
| SwabExpress (USA) | RT-PCR | S, ORF 1b | LOD: 2–4 RNA/μL (sensitivity: 100%) | 99.4% | [168] |
| PrimeDirect® Probe RT-qPCR Mix (Takara) | RT-PCR | E, RdRp | 55.1–91.9% | 88% | [169] |
| PrimeScript®RT-PCR (Takara) | RT-PCR | E, RdRp | 69.6–89.2% | 100% | [169] |
| ViroReal® Kit RT-LAMP SARS-CoV-2 (Ingenetix) | RT-LAMP | ORF 1ab | LOD: 100 RNA copies/reaction (95%) | 99% | [175] |
| CASSPIT (India) | CRISPR/Cas | S, ORF 1ab | LOD: 100 RNA copies/reaction (~100%) | 100% | [176] |
| QSanger™-Covid-19 Assay (Swift Biosciences) | Nucleic acid sequencing | Unclear | LOD: 3200 RNA copies/mL | Unclear | [177] |