Table 2.
Overview of studies testing the efficacy of anti-microbial packaging with essential oils and plant extracts or their components on meat products.
| Anti-Microbial Agent—Concentration Applied | Food Product | Packaging Material | Microorganisms Targeted | Indicative Reduction (Log10 CFU/g, Log10 CFU/mL, Log10 CFU/cm2) 1,2,3,4 | Reference |
|---|---|---|---|---|---|
| Angelica Root—0.1% v/w | Pork | Polyethylene (PE) film | Total counts | 2.34 | [51] |
| Brochothrix thermosphacta | 1.49 | ||||
| Carnobacterium spp. | 1.04 | ||||
| Enterobacteriaceae | 1.95 | ||||
| Staphylococcus sp. | 1.17 | ||||
| Pseudomonas sp. | 0.89 | ||||
| Enterococcus sp. | 0.81 | ||||
| Chrysanthemum—1.5% w/v | Beef | Chitosan/nanofibers film | L. monocytogenes | 1.2–1.5 | [52] |
| Cinnamon—10% w/w | Pork | Polylactic acid (PLA)/b-cyclodextrin nanofilm | Total viable counts | 4.5 5 | [53] |
| 5 mg/mL | Chicken | Sodium alginate (NaAlg) coating | Total viable counts | 1.4 | [54] |
| Psychrotrophic bacteria | 1 | ||||
| Pseudomonas spp. | 0.8 | ||||
| Enterobacteriaceae | 0.6 | ||||
| Lactic acid bacteria | 1.5 | ||||
| Yeasts and moulds | 0.5 | ||||
| L. monocytogenes | 1 | ||||
| Clove—0.1% v/w | Pork | Polyethylene (PE) film | Total counts | 2.49 | [51] |
| B. thermosphacta | 2.17 | ||||
| Carnobacterium spp. | 1.62 | ||||
| Enterobacteriaceae | 2.37 | ||||
| Staphylococcus sp. | 1.73 | ||||
| Pseudomonas sp. | 1.34 | ||||
| Enterococcus sp. | 1.26 | ||||
| 3% | Ground chicken meat | Carboxymethyl cellulose–polyvinyl alcohol (CMC-PVOH) film | Total viable counts | 3 6 | [55] |
| 1, 2 mg/g | Beef | Chitosan-myristic acid nanogel coating | Salmonella enterica ser Enteritidis | 0.3–0.8 | [56] |
| 0.5 g in 9 × 5 cm film | Ground chicken | Linear-low density polyethylene (LLDPE) film | S. Typhimurium | 7 | [57] |
| L. monocytogenes | 6 | ||||
| Coriander—0.5%, 1% | Chicken | Sodium alginate (NaAlg)-glycerol coating | Mesophilic bacteria | 1–1.5 | [58] |
| Psychrotrophic bacteria | 0.8–1.2 | ||||
| Lactic acid bacteria | 1.3–2.3 | ||||
| Coliforms | 1.4–2.4 | ||||
| Staphylococcus aureus | 1.2–2.1 | ||||
| Moulds and yeasts | 1.1–1.5 | ||||
| Cumin—Black zira—0.5%, 1% v/v | Ground beef | Polylactic acid (PLA)-nanocellulose (NC) film | Total viable bacteria | 0.7 | [59] |
| Lactic acid bacteria | 0.1–0.2 | ||||
| Enterobacteriaceae | 0.5–0.8 | ||||
| Psychrotrophic bacteria | 0.2–0.3 | ||||
| S. aureus | 0.6–1.2 | ||||
| Pseudomonas spp. | 0.1–0.3 | ||||
| 0.5%, 1%, 2% v/v | Chicken | Chitosan coating | Mesophilic bacteria | 0.45–0.59 | [60] |
| Psychrotrophic bacteria | 0 | ||||
| Lactic acid bacteria | 0 | ||||
| Enterobacteriaceae | 0 | ||||
| Yeasts and moulds | 0 | ||||
| Eucalyptus—0.5%, 1%, 2% v/v | Chicken | Chitosan coating | Mesophilic bacteria | 0–0.69 | [60] |
| Psychrotrophic bacteria | 1.11–1.41 | ||||
| Lactic acid bacteria | 0 | ||||
| Enterobacteriaceae | 0 | ||||
| Yeasts and moulds | 0 | ||||
| Garlic—2%, 4%, 6%, 8% w/w | Ready-to-Eat (RTE) beef loaf | Low density polyethylene (LDPE) film | L. monocytogenes | 0.2–0.5 | [61] |
| E. coli | 0–0.2 | ||||
| B. thermosphacta | 0–0.2 | ||||
| Horseradish, mustard—0.6, 1.2 µg/h release rate | Chicken | Glass vial inside high density polyethylene (HDPE) film | S. enterica ser Typhimurium | 1–1.2 | [62] |
| L. monocytogenes | 0.1–0.6 | ||||
| Total aerobic bacteria | 0.5–1 | ||||
| 20%, 28%, 40%, 52%, 58% v/w | Cooked chicken | Cellulose film with carbon nanotube (CNT) | Salmonella Choleraesuis | 2.5–8 | [63] |
| Psychrotrophic bacteria | 1.5–8 | ||||
| Mesophilic bacteria | 0.7–8 | ||||
| Lemongrass—2% | Pork sausage | Polylactic acid (PLA) film | L. monocytogenes | 1.47 | [64] |
| Nutmeg—0.5%, 1%, 1.5%, 2% | Beef | Sage seed mucilage coating | Total viable count | 3–8 | [65] |
| Psychrotrophic bacteria | 1.8–5.5 | ||||
| E. coli | 0.5–2 | ||||
| S. aureus | 1–3 | ||||
| Yeasts and moulds | 1–5 | ||||
| Oregano—1%, 2%, 3% v/v | Beef | Soy protein coating | E. coli O157:H7 | 1.2–1.83 | [66] |
| L. monocytogenes | 0.9–1.9 | ||||
| S. aureus | 1.8–2.86 | ||||
| 5% v/v | Ground beef patties | Soy protein film | Total viable count | 0 | [67] |
| Lactic acid bacteria | 0 | ||||
| Staphylococcus spp. | 0 | ||||
| Pseudomonas spp. | 0.6 | ||||
| Coliforms | 0.5 | ||||
| Pepper—2% w/w | Chicken | Carboxymethyl cellulose (CMC) coating | Mesophilic bacteria | 1.5–2.2 | [68] |
| Psychrotrophic bacteria | 1.5–2 | ||||
| Peppermint—0.5%, 1% v/v | Ground beef | Polylactic acid (PLA)- nanocellulose (NC) film | Total viable bacteria | 0.7 | [59] |
| Lactic acid bacteria | 0–0.4 | ||||
| Enterobacteriaceae | 0.6–0.8 | ||||
| Psychrotrophic bacteria | 0.4 | ||||
| S. aureus | 0.6–0.8 | ||||
| Pseudomonas spp. | 0.1–0.4 | ||||
| Rosemary—4% w/w | Chicken | 3 layers film (paper-metallic layer- high density polyethylene (HDPE)) | Mesophilic bacteria | 0.3 | [69] |
| Enterobacteriaceae | 0 | ||||
| Pseudomonas spp. | 0.24 | ||||
| B. thermosphacta | 0.37 | ||||
| 5 mg/mL | Chicken | Sodium alginate (NaAlg) coating | Total viable counts | 1.6 | [54] |
| Psychrotrophic bacteria | 1.6 | ||||
| Pseudomonas spp. | 1.3 | ||||
| Enterobacteriaceae | 1.6 | ||||
| Lactic acid bacteria | 1.7 | ||||
| Yeasts and moulds | 0.8 | ||||
| L. monocytogenes | 1.5 | ||||
| 0.1% v/w | Pork | Polyethylene (PE) film | Total counts | 2.53 | [51] |
| B. thermosphacta | 1.95 | ||||
| Carnobacterium spp. | 1.5 | ||||
| Enterobacteriaceae | 2.48 | ||||
| Staphylococcus sp. | 1.39 | ||||
| Pseudomonas sp. | 1.08 | ||||
| Enterococcus sp. | 0.98 | ||||
| 4% w/w | Beef | 3 layers film (paper-metallic layer- high density polyethylene (HDPE)) | Psychrotrophic bacteria | 0–0.7 7 | [70] |
| B. thermosphacta | 0–1.5 7 | ||||
| Pseudomonas spp. | 0–0.8 7 | ||||
| Enterobacteriaceae | 0–0.8 7 | ||||
| Thyme—1%, 2%, 3% v/v | Beef | Soy protein coating | E. coli O157:H7 | 1.2–1.87 | [66] |
| L. monocytogenes | 1–1.97 | ||||
| S. aureus | 1.6–2.6 | ||||
| 5% v/v | Ground beef patties | Soy protein film | Total viable count | 0 | [67] |
| Lactic acid bacteria | 0 | ||||
| Staphylococcus spp. | 0.2 | ||||
| Pseudomonas spp. | 0.9 | ||||
| Coliforms | 0.6 | ||||
| 1% | Sausage | Chitosan coating | Yeasts and moulds | 0.6–1.5 | [71] |
| Turmeric—2% w/w | Chicken | Carboxymethyl cellulose (CMC) coating | Mesophilic bacteria | 2.2–3 | [68] |
| Psychrotrophic bacteria | 3.5 |
The synergistic effect of anti-microbial agents or their component, where it has been studied, is not cited. 1 The difference of population that is cited is sometimes an approximate number, because of lack of exact data; 2 When the range of population difference is cited; it corresponds to the different experimental conditions studied (e.g., different concentrations, storage temperatures, etc.); 3 When the anti-microbial agents were combined with other preservation methods or were incorporated into chitosan coatings, the effect of the agent alone is cited; 4 These classifications apply to the end point of each experiment; 5 The population difference cited corresponds to the 4th day of the experiment, because of lack of data for the pork packed with the fresh-keeping film; 6 The population difference cited corresponds to the 4th day of the experiment, because of lack of data for the control sample of ground chicken; 7 The population difference cited corresponds to the 10th day of the experiment, because of lack of data for the beef stored at aerobic conditions.