Table 1.
Average (n = 3) | |||
---|---|---|---|
Number of Human GE | Cq (Classical) | Cq (Non-Competitive #A) | Cq (Non-Competitive #B) |
10,000–100,000 | 25.3 | 29.2 | 33.2 |
1000–10,000 | 28.7 | 32.4 | 35.5 |
100–1000 | 31.5 | 36.1 | 37.5 |
10–100 | 35.2 | 39.4 | n.d. |
1–10 | 38.1 | n.d. | n.d. |
Performance difference between classical (RNase P-based) SAC assay [1,3,12,13,36] and non-competitive SAC assays; Legend: The RNase P assay is performed as described (see [1] and referenced work). The calibration between human genome equivalents and Cq values is estimated using dilutions of known human genomic DNA standards.