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. 2021 Jun 22;11(7):1133. doi: 10.3390/diagnostics11071133

Table 1.

Relation between Cq values of classical SAC assay and non-competitive SAC assay: note a shift of qPCR product appearance (of non-competitive SAC) toward high Cq values. This is allowing detection of low pathogen-positive signals, and offering confidence in negative result.

Average (n = 3)
Number of Human GE Cq (Classical) Cq (Non-Competitive #A) Cq (Non-Competitive #B)
10,000–100,000 25.3 29.2 33.2
1000–10,000 28.7 32.4 35.5
100–1000 31.5 36.1 37.5
10–100 35.2 39.4 n.d.
1–10 38.1 n.d. n.d.

Performance difference between classical (RNase P-based) SAC assay [1,3,12,13,36] and non-competitive SAC assays; Legend: The RNase P assay is performed as described (see [1] and referenced work). The calibration between human genome equivalents and Cq values is estimated using dilutions of known human genomic DNA standards.