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. 2021 Jul 3;9(7):1438. doi: 10.3390/microorganisms9071438

Figure 1.

Figure 1

SARS-CoV-2 does not productively infect HL-mECs. (A) Vero E6 and HL-mECs were infected with SARS-CoV-2 at a MOI of 0.05 or 1. The graph shows SARS-CoV-2 genome quantitation in cell supernatants collected at 1, 3, 6, 12, 24, 48, and 72 h p.i. by qRT-PCR. Values represent the S gene copies/mL mean ± SD of a triplicate. (B) Supernatants were collected from Vero E6 and HL-mECs at 1, 3, 6, 12, 24, 48, and 72 h p.i. and infectious viral titers were determined by plaque assay. Data represent the average of three replicates from one experiment. (C) Deep sequencing analysis of SARS-CoV-2 viral progeny released from HL-mECs or (D) from Vero E6 cells at day 3 p.i. Whole-genome sequences of SARS-CoV-2 released in the supernatants were performed by metagenomic analyses. Obtained reads were mapped on the virus genome reference NC_045512.2 (x-axis) for genome reconstruction; the y-axis shows the number of reads mapped at each nucleotide position.