Figure 9.

The effect of complexes 2 and 5 on the activation of MAPKs. THP-1 cells were differentiated to macrophages by PMA. The cells were pre-treated by complexes 2 and 5 (200 nM) for 1 h, then LPS (1 µg/mL) was added to trigger the inflammatory response. Cells were collected 30 min after LPS stimulation. The amount of proteins was detected by Western blot and immunodetection. Present immunoblots show the results of pooled four independent reactions. Arrows indicate the correct position of particular proteins.