Figure 12.

Nuclease-like effect of the tested compounds. Supercoiled plasmid DNA (CCC) was incubated with complexes 1, 2, and 4, CuSO₄·5H₂O, pomiferin (Pom.) or pure solvent (10% DMF in water) in different concentrations at 37 °C for 1 h in water (A) or in 0.66 mM H₂O₂ (B). After incubation, the amount of created open circle (OC) and linear (L) form of plasmid was evaluated by densitometry analysis. Graphs show means ± SEM of four independent experiments, electrophoretograms represent the results of agarose electrophoresis. * indicates statistical difference to 10% DMF group (p ≤ 0.05); ** indicates statistical difference to 10% DMF group (p ≤ 0.01); *** indicates statistical difference to 10% DMF group (p ≤ 0.001); **** indicates statistical difference to DMF group (p ≤ 0.0001). L control linearized plasmid pUC19. N. D. = not detected. CuSO₄ stands for water solution of copper(II) sulphate pentahydrate.