Figure 13.

Nuclease-like effect of the tested compounds in the presence of OH^• scavengers DMSO (A), O₂^•- scavengers KI (B), and ¹O₂ scavengers NaN₃ (C). Supercoiled plasmid DNA (CCC) was incubated with complexes 1, 2, and 4, pomiferin (Pom.) in different concentrations or pure solvent (DMF) for 1 h at 37 °C in the 0.66 mM H₂O₂. Scavengers were applied together with tested complexes in equimolar concentration. After incubation, the amount of created open circle (OC) and linear (L) forms of plasmid was evaluated by densitometric analysis. Graphs indicate means ± SEM of four independent experiments, electrophoretograms represent representative results of agarose electrophoresis. * indicates statistical difference between indicated groups (p ≤ 0.05); ** indicates statistical difference between indicated groups (p ≤ 0.01); *** indicates statistical difference between indicated groups (p ≤ 0.001); **** indicates statistical difference between indicated groups (p ≤ 0.0001). N. D. not detected.