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. 2021 Jul 14;22(14):7540. doi: 10.3390/ijms22147540

Figure 1.

Figure 1

Dose-dependent cytokine responses of human PBMC induced by SARS CoV-2 S1-Fc fusion protein. Rested PBMC from two healthy donors (HD1 and HD2) were cultured for 48 h with 0, 0.4, 1.0 and 2.5 µg/mL of S1-Fc purchased from Vendor #1 (lot 24056-2002-2). The levels of IL-1β, IL-6, IL-8, IL-10, IL-12 and TNFα in the supernatants of cultured PBMC were assessed using the CBA human inflammatory cytokine kit and flow cytometric analysis. Data shown are representative of the flow cytometric results from at least two separate experiments performed on samples from healthy donors, HD1 and HD2.