Table 1.
(A): Mean diameter of inhibition zones (mm) for E. coli (ETEC) growth inhibited by alkaloid rich fraction of Holarrhena pubescens (kutaj). (B): Disc diffusion test for antimicrobial activity of Holarrhena pubescens (kutaj): (a) Zone of inhibition of positive control (gentamycin), (b) Zone of inhibition of alkaloid fraction, (c) Zone of inhibition of negative control.
| Enterotoxigenic E. coli (ETEC) | ||||
|---|---|---|---|---|
| A | B | |||
| Treatment | Concentration | Dose/Disc | Zone of Inhibition |
|
| Alkaloid Rich Fraction (mg/mL) | 100 mg/mL | 1 mg | 16 ± 0.38 mm | |
| 50 mg/mL | 0.5 mg | 14 ± 0.53 mm | ||
| 25 mg/mL | 0.25 mg | 0.0 ± 0.0 | ||
| 12.5 mg/mL | 0.125 mg | 0.00 ± 0.0 | ||
| 6.25 mg/mL | 0.625 mg | 0.00 ± 0.0 | ||
| Positive control (Gentamycin) | 10 µg | 35 ± 0.707 mm | ||
| Negative Control | - | Nil | ||
| Solvent Control | - | Nil | ||